Signaling networks transported by EVs drive phenocopying of migratory behavior between tumor cells

ABSTRACT: Tumors are heterogeneous with respect to mutational pattern, gene expression and microenvironment. This heterogeneity leads to large functional intratumoral differences in tumor cell behavior so that a tumor may contain or develop small fractions of cells that possess properties to migrate, metastasize or evade therapy treatment. In recent years, extracellular vesicles (EVs) have attracted a lot of attention as microenvironmental intercellular messenger that may severely complicate tumor heterogeneity. EVs are small lipid membrane-enclosed vesicles that carry biologically active molecules including lipids, proteins, DNA and various RNA species. Although accumulating evidence suggest that tumor cells can phenocopy behavior through exchange of EVs, it is widely debated how the transfer of small amounts of cargo can mediate this effect. We isolated EVs from tumors grown in mice and identified that sub tumor clones with distinct metastatic potential transfer networks of RNAs and proteins involved in migration, and leads to phenocopying of migratory behavior.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Melanocyte, Whole Body

SUBMITTER: Sander Piersma  

LAB HEAD: Connie Ramona Jimenez

PROVIDER: PXD006439 | Pride | 2018-06-22

REPOSITORIES: Pride