Dataset Information

Predicting species-resolved macronutrient acquisition during succession in a model phototrophic biofilm

ABSTRACT: Proteomic characterization of a cyanobacterial micorbial community isolated from a merimetic lake

OTHER RELATED OMICS DATASETS IN: PRJNA387588

INSTRUMENT(S):

ORGANISM(S): Synechocystis Sp. Pcc 6803

SUBMITTER: Matthew Monroe

LAB HEAD: Mary S. Lipton

PROVIDER: PXD006440 | Pride | 2017-07-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	HLP_Oscar_D14P01_6Jun14_Tiger_14-03-03.mgf	Mgf
	HLP_Oscar_D14P01_6Jun14_Tiger_14-03-03.pride.mgf.gz	Mgf
	HLP_Oscar_D14P01_6Jun14_Tiger_14-03-03.raw	Raw
	HLP_Oscar_D14P01_6Jun14_Tiger_14-03-03_msgfplus.mzid.gz	Mzid
	HLP_Oscar_D14P01_6Jun14_Tiger_14-03-03_msgfplus.pride.mztab.gz	Mztab

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Publications

Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated 'Omics Approach.

Lindemann Stephen R SR Mobberley Jennifer M JM Cole Jessica K JK Markillie L M LM Taylor Ronald C RC Huang Eric E Chrisler William B WB Wiley H S HS Lipton Mary S MS Nelson William C WC Fredrickson James K JK Romine Margaret F MF

Frontiers in microbiology 20170613

The principles governing acquisition and interspecies exchange of nutrients in microbial communities and how those exchanges impact community productivity are poorly understood. Here, we examine energy and macronutrient acquisition in unicyanobacterial consortia for which species-resolved genome information exists for all members, allowing us to use multi-omic approaches to predict species' abilities to acquire resources and examine expression of resource-acquisition genes during succession. Met ...[more]

PMID: 28659875

Similar Datasets

Project description:Microbial autotroph-heterotroph interactions influence biogeochemical cycles on a global scale, but the diversity and complexity of natural systems and their intractability to in situ manipulation make it challenging to elucidate the principles governing these interactions. The study of assembling phototrophic biofilm communities provides a robust means to identify such interactions and evaluate their contributions to the recruitment and maintenance of phylogenetic and functional diversity overtime. To examine primary succession in phototrophic communities, we isolated two unicyanobacterial consortia from the microbial mat in HotLake, Washington, characterizing the membership and metabolic function of each consortium. We then analyzed the spatial structures and quantified the community compositions of their assembling biofilms. The consortia retained the same suite of heterotrophic species, identified as abundant members of the mat and assigned to Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes. Autotroph growth rates dominated early in assembly, yielding to increasing heterotroph growth rates late in succession. The two consortia exhibited similar assembly patterns, with increasing relative abundances of members from Bacteroidetes and Alphaproteobacteria concurrent with decreasing relative abundances of those from Gamma proteobacteria. Despite these similarities at higher taxonomic levels, the relative abundances of individual heterotrophic species were substantially different in the developing consortial biofilms. This suggests that, although similar niches are created by the cyanobacterial metabolisms, the resulting webs of autotroph-heterotroph and heterotroph-heterotroph interactions are specific to each primary producer. The relative simplicity and tractability of the Hot Lake unicyanobacterial consortia make them useful model systems for deciphering interspecies interactions and assembly principles relevant to natural microbial communities.