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HLA-C05:01 and HLA-C07:02 from 721.221 cells

ABSTRACT: HLA-C expresion varies widely across the different HLA-C alleles. MicroRNA binding can partly explain the differences in HLA-C allele expression however other contributing factors still remain undetermined. Here we use two common HLA-C alleles, HLA-C*05:01 and HLA-C*07:02, to explore differences in expression levels. Using functional, structural and peptide repertoire comparisons we demonstrate that HLA-C expression levels are not only modulated at the RNA level but also at the protein level. This dataset contains RAW data and database search results for HLA-C*05:01 and HLA-C*07:02 from the 721.221 cell line.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Cell Culture

SUBMITTER: Jesse Mobbs

LAB HEAD: Anthony W Purcell

PROVIDER: PXD006455 | Pride | 2017-07-03

REPOSITORIES: Pride

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Structural and regulatory diversity shape HLA-C protein expression levels.

Kaur Gurman G Gras Stephanie S Mobbs Jesse I JI Vivian Julian P JP Cortes Adrian A Barber Thomas T Kuttikkatte Subita Balaram SB Jensen Lise Torp LT Attfield Kathrine E KE Dendrou Calliope A CA Carrington Mary M McVean Gil G Purcell Anthony W AW Rossjohn Jamie J Fugger Lars L

Nature communications 20170626

Expression of HLA-C varies widely across individuals in an allele-specific manner. This variation in expression can influence efficacy of the immune response, as shown for infectious and autoimmune diseases. MicroRNA binding partially influences differential HLA-C expression, but the additional contributing factors have remained undetermined. Here we use functional and structural analyses to demonstrate that HLA-C expression is modulated not just at the RNA level, but also at the protein level. ...[more]

PMID: 28649982

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