Proteomics

Dataset Information

0

Bering Strait surface water and Chukchi Sea bottom water microbiome metaproteomics


ABSTRACT: Analysis of bacterial fraction collected on GF/F filters post pre-filtration on 1um filter. 15L were filtered from Bering Strait (BSt) surface water and Chukchi Sea (station 2) bottom waters.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Marine Metagenome

TISSUE(S): Cell Suspension Culture

SUBMITTER: Brook Nunn  

LAB HEAD: Brook L. Nunn

PROVIDER: PXD006472 | Pride | 2019-01-25

REPOSITORIES: Pride

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Publications

An Alignment-Free "Metapeptide" Strategy for Metaproteomic Characterization of Microbiome Samples Using Shotgun Metagenomic Sequencing.

May Damon H DH   Timmins-Schiffman Emma E   Mikan Molly P MP   Harvey H Rodger HR   Borenstein Elhanan E   Nunn Brook L BL   Noble William S WS  

Journal of proteome research 20160719 8


In principle, tandem mass spectrometry can be used to detect and quantify the peptides present in a microbiome sample, enabling functional and taxonomic insight into microbiome metabolic activity. However, the phylogenetic diversity constituting a particular microbiome is often unknown, and many of the organisms present may not have assembled genomes. In ocean microbiome samples, with particularly diverse and uncultured bacterial communities, it is difficult to construct protein databases that c  ...[more]

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