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A plasmid goes viral, part 2 - A plasmid from an Antarctic haloarchaeon uses specialized membrane vesicles to disseminate and infect plasmid-free cells

ABSTRACT: Analysis of protein compostition of plasmid vesicles (PVs) purified through CsCl gradients.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Halorubrum Lacusprofundi Atcc 49239

TISSUE(S): Cell Culture

SUBMITTER: Susanne Erdmann

LAB HEAD: Ricardo Cavicchioli

PROVIDER: PXD006515 | Pride | 2017-07-14

REPOSITORIES: Pride

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A plasmid from an Antarctic haloarchaeon uses specialized membrane vesicles to disseminate and infect plasmid-free cells.

Erdmann Susanne S Tschitschko Bernhard B Zhong Ling L Raftery Mark J MJ Cavicchioli Ricardo R

Nature microbiology 20170821 10

The major difference between viruses and plasmids is the mechanism of transferring their genomic information between host cells. Here, we describe the archaeal plasmid pR1SE from an Antarctic species of haloarchaea that transfers via a mechanism similar to a virus. pR1SE encodes proteins that are found in regularly shaped membrane vesicles, and the vesicles enclose the plasmid DNA. The released vesicles are capable of infecting a plasmid-free strain, which then gains the ability to produce plasm ...[more]

PMID: 28827601

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