A thiol probe for measuring unfolded protein load and proteostasis in cells

ABSTRACT: When proteostasis becomes unbalanced, unfolded proteins accumulate and can aggregate. However, probes quantifying proteostasis imbalance are lacking. We report a dye, tetraphenylethene maleimide (TPE-MI) that can measure unfolded protein load.TPE-MI fluorescence is enhanced upon reaction with cellular proteomes under conditions promoting accumulation of unfolded proteins and, via proteomic analysis, can track which proteins expose more cysteine residues under stress.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Nagaraj Moily  

LAB HEAD: Danny Hatters

PROVIDER: PXD006527 | Pride | 2018-10-24

REPOSITORIES: Pride