Interactome of Ca2+-bound secretagogin in INS-1E cells
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ABSTRACT: By using His6-tagged recombinant human secretagogin in the presence of Ca2+ (100 µM), we precipitated putative interacting partners from INS-1E cells and identified their amino acid sequences by mass spectrometry. We detected canonical interacting proteins participating in vesicle-mediated transport, exocytosis and cytoskeletal organization. Besides, we captured proteins controlling protein folding and (de-)ubiquitination. Our data support that secretagogin modulates protein folding and degradation through protein-protein interactions and interacts with USP9X in β cells to control cell survival.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Rattus Norvegicus (rat)
TISSUE(S): Type B Pancreatic Cell, Immortal Cell Line Cell, Cell Culture
SUBMITTER: Edit Szodorai
LAB HEAD: Univ. Prof. Dr. Tibor Harkany
PROVIDER: PXD006544 | Pride | 2017-06-21
REPOSITORIES: Pride
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