Sequential Peptide Immunopurification Methodology to Monitor the Crosstalk between SUMOylation and Ubiquitylation

ABSTRACT: We describe a method that permits the identification of proteins that are modified by both SUMOylation and ubiquitylation to better understand the role of this crosstalk. The procedure requires 3 days when starting from cell pellets and can yield more than 8000 SUMO sites and 3500 ubiquitin sites from 16 mg of cell extract.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Francis McManus  

LAB HEAD: Pierre Thibault

PROVIDER: PXD006545 | Pride | 2018-03-27

REPOSITORIES: Pride