Dataset Information

A comprehensive Proteomic Investigation of Ebf1 Heterozygosity in pro B lymphocytes utilizing Data Independent Acquisition (DIA) and Data Dependent Acquisition (DDA) Approaches

ABSTRACT: The study provide a comprehensive comparison between data dependent acquistion (DDA) and data independen acquisition (DIA) with the aim to detect and quantify proteome changes associated with reduced gene dosage using Ebf1 transcription factor as a modal.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): B Cell, Blood

SUBMITTER: Yaarub Musa

LAB HEAD: Gerhard Mittler

PROVIDER: PXD006620 | Pride | 2022-03-21

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 13

Publications

Comprehensive Proteomic Investigation of Ebf1 Heterozygosity in Pro-B Lymphocytes Utilizing Data Independent Acquisition.

Musa Yaarub R YR Boller Sören S Puchalska Monika M Grosschedl Rudolf R Mittler Gerhard G

Journal of proteome research 20171211 1

Early B cell factor 1 (EBF1) is one of the key transcription factors required for orchestrating B-cell lineage development. Although studies have shown that Ebf1 haploinsufficiency is involved in the development of leukemia, no study has been conducted that characterizes the global effect of Ebf1 heterozygosity on the proteome of pro-B lymphocytes. Here, we employ both data independent acquisition (DIA) and shotgun data dependent acquisition (DDA) workflows for profiling proteins that are differ ...[more]

PMID: 29181981

Similar Datasets

Project description:ObjectiveTo identify cognitive phenotypes in temporal lobe epilepsy (TLE) and test their reproducibility in a large, multi-site cohort of patients using both data-driven and clinically driven approaches.MethodFour-hundred seven patients with TLE who underwent a comprehensive neuropsychological evaluation at one of four epilepsy centers were included. Scores on tests of verbal memory, naming, fluency, executive function, and psychomotor speed were converted into z-scores based on 151 healthy controls (HCs). For the data-driven method, cluster analysis (k-means) was used to determine the optimal number of clusters. For the clinically driven method, impairment was defined as >1.5 standard deviations below the mean of the HC, and patients were classified into groups based on the pattern of impairment.ResultsCluster analysis revealed a three-cluster solution characterized by (a) generalized impairment (29%), (b) language and memory impairment (28%), and (c) no impairment (43%). Based on the clinical criteria, the same broad categories were identified, but with a different distribution: (a) generalized impairment (37%), (b) language and memory impairment (30%), and (c) no impairment (33%). There was a 82.6% concordance rate with good agreement (κ = .716) between the methods. Forty-eight patients classified as having a normal profile based on cluster analysis were classified as having generalized impairment (n = 16) or an isolated language/memory impairment (n = 32) based on the clinical criteria. Patients with generalized impairment had a longer disease duration and patients with no impairment had more years of education. However, patients demonstrating the classic TLE profile (ie, language and memory impairment) were not more likely to have an earlier age at onset or mesial temporal sclerosis.SignificanceWe validate previous findings from single-site studies that have identified three unique cognitive phenotypes in TLE and offer a means of translating the patterns into a clinical diagnostic criteria, representing a novel taxonomy of neuropsychological status in TLE.

			Action	DRS
	DDA_Het_R01.raw	Raw
	DDA_Het_R02.raw	Raw
	DDA_Het_R03.raw	Raw
	DDA_WA_R01.raw	Raw
	DDA_WA_R02.raw	Raw