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Shift of EMT gradient in 3D spheroid MSCs for activation of mesenchymal niche function

ABSTRACT: This project is described about the detailed characterization of 3D spheroid mesenchymal stem cells (MSCs). Specifically, authors showed the dynamic action towards EMT in 3D MSCs and the potential of EMT-enhanced “naïve” mesenchymal phenotype.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Jihye Shin

LAB HEAD: Cheolju Lee

PROVIDER: PXD006653 | Pride | 2018-06-25

REPOSITORIES: Pride

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Shift of EMT gradient in 3D spheroid MSCs for activation of mesenchymal niche function.

Jeon Sohee S Lee Ho-Sun HS Lee Ga-Young GY Park Gyeongsin G Kim Tae-Min TM Shin Jihye J Lee Cheolju C Oh Il-Hoan IH

Scientific reports 20170731 1

Despite the wide use of mesenchymal stromal cells (MSCs) for paracrine support in clinical trials, their variable and heterogeneous supporting activity pose major challenges. While three-dimensional (3D) MSC cultures are emerging as alternative approaches, key changes in cellular characteristics during 3D-spheroid formation remain unclear. Here, we show that MSCs in 3D spheroids undergo further progression towards the epithelial-mesenchymal transition (EMT), driven by upregulation of EMT-promoti ...[more]

PMID: 28761088

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