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Iron response of HepG2 cells - The exon-intron gene structure upstream of the initiation codon predicts translation efficiency

ABSTRACT: Quantitative proteomics of HepG2 cells in response to iron. SILAC 3plex was conducted

OTHER RELATED OMICS DATASETS IN: PRJNA389312

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Chun Shen Lim

LAB HEAD: Chris Brown

PROVIDER: PXD006661 | Pride | 2018-04-23

REPOSITORIES: Pride

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The exon-intron gene structure upstream of the initiation codon predicts translation efficiency.

Lim Chun Shen CS T Wardell Samuel J SJ Kleffmann Torsten T Brown Chris M CM

Nucleic acids research 20180501 9

Introns in mRNA leaders are common in complex eukaryotes, but often overlooked. These introns are spliced out before translation, leaving exon-exon junctions in the mRNA leaders (leader EEJs). Our multi-omic approach shows that the number of leader EEJs inversely correlates with the main protein translation, as does the number of upstream open reading frames (uORFs). Across the five species studied, the lowest levels of translation were observed for mRNAs with both leader EEJs and uORFs (29%). T ...[more]

PMID: 29684192

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