Proteomic characterization of transcription and splicing factors associated with a more invasive phenotype in colorectal cancer

ABSTRACT: To find new transcription and splicing factors associated with the metastatic phenotype we used a concatenated tandem array of consensus transcription factors response elements (catTFRE) on nuclear extracts of colon cancer cell lines. We used two different isogenic pairs of colorectal metastatic cells, KM12SM/KM12C and SW620/480. Proteins were pulled-down using biotinylated DNA sequences and streptavidin beads and analyzed by LC-MSMS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Consuelo Marín-Vicente  

LAB HEAD: J. Ignacio Casal Alvarez

PROVIDER: PXD006662 | Pride | 2017-11-15

REPOSITORIES: Pride