Proteomics

Dataset Information

0

Specter - Specter: linear deconvolution for targeted analysis of data-independent acquisition mass spectrometry proteomics


ABSTRACT: These data cover all of the analyses described in the paper "Specter: linear deconvolution as a new paradigm for targeted analysis of data-independent acquisition mass spectrometry proteomics". Specifically, the data consist of - 20 DIA and DDA files from the HEK293T/synthetic phosphopeptides spike-in experiments - 10 DDA files, one for each of ten fractions of an E. coli lysate digest - 14 DIA files for the experiments involving mixtures of synthetic peptides - 11 DDA files for the isolated runs of these synthetic peptides - 84 DIA files for measurements of the phosphoproteome of perturbed PC3 cells - 10 DDA files for spectral library construction for the phosphoproteomics data - 3 DIA and 3 DDA files for analysis of an unfractionated E. coli lysate digest. See the spreadsheet "Specter Datasets Catalog.xlsx" for further descriptions and file metadata.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Cell Culture, Prostate Cancer Cell Line

SUBMITTER: Ryan Peckner  

LAB HEAD: Steven Carr

PROVIDER: PXD006722 | Pride | 2018-03-19

REPOSITORIES: Pride

altmetric image

Publications

Specter: linear deconvolution for targeted analysis of data-independent acquisition mass spectrometry proteomics.

Peckner Ryan R   Myers Samuel A SA   Jacome Alvaro Sebastian Vaca ASV   Egertson Jarrett D JD   Abelin Jennifer G JG   MacCoss Michael J MJ   Carr Steven A SA   Jaffe Jacob D JD  

Nature methods 20180402 5


Mass spectrometry with data-independent acquisition (DIA) is a promising method to improve the comprehensiveness and reproducibility of targeted and discovery proteomics, in theory by systematically measuring all peptide precursors in a biological sample. However, the analytical challenges involved in discriminating between peptides with similar sequences in convoluted spectra have limited its applicability in important cases, such as the detection of single-nucleotide polymorphisms (SNPs) and a  ...[more]

Similar Datasets

2016-09-28 | PXD004558 | Pride
2018-05-01 | PXD008029 | Pride
2021-09-30 | PXD020195 | Pride
2019-10-21 | PXD009597 | Pride
2020-05-12 | PXD019077 | Pride
2020-04-07 | PXD010943 | Pride
2021-09-13 | PXD020283 | Pride
2019-04-25 | PXD009246 | Pride
2015-02-04 | PXD000672 | Pride
2015-01-21 | PXD001587 | Pride