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Quantitative Proteome-level Analysis of Paulownia Witches’ Broom Disease with Methyl Methane Sulfonate Assistance Reveals Diverse Metabolic Changes During the Infection and Recovery Processes

ABSTRACT: A total of 2358 proteins were identified. We investigated the proteins profiles in PF vs. PFI (infected process) and PFI-20 vs. PFI-60 (recovered process), and further found that many of the MMS-response proteins mapped to “photosynthesis” and “ribosome” pathways. Based on our comparison scheme, 36 PaWB-related proteins were revealed.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Paulownia Fortunei

SUBMITTER: zhou lianqi

LAB HEAD: Guoqiang Fan

PROVIDER: PXD006731 | Pride | 2017-06-27

REPOSITORIES: Pride

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Quantitative proteome-level analysis of paulownia witches' broom disease with methyl methane sulfonate assistance reveals diverse metabolic changes during the infection and recovery processes.

Wang Zhe Z Liu Wenshan W Fan Guoqiang G Zhai Xiaoqiao X Zhao Zhenli Z Dong Yanpeng Y Deng Minjie M Cao Yabing Y

PeerJ 20170703

Paulownia witches' broom (PaWB) disease caused by phytoplasma is a fatal disease that leads to considerable economic losses. Although there are a few reports describing studies of PaWB pathogenesis, the molecular mechanisms underlying phytoplasma pathogenicity in Paulownia trees remain uncharacterized. In this study, after building a transcriptome database containing 67,177 sequences, we used isobaric tags for relative and absolute quantification (iTRAQ) to quantify and analyze the proteome-leve ...[more]

PMID: 28690927

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