Dataset Information

Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract – a role for antioxidants in skin health

ABSTRACT: Environmental air pollution such as diesel smoke emission has been shown to have an adverse effect on human skin. Although epidemiological studies showing adverse effects such as oxidative stress-induced aging exists, studies showing the cellular and molecular response to such stress are rare. Using a primary skin keratinocyte model and TMT-based quantitative proteomics strategy, we studied the effects of chronic exposure to diesel particulate matter (DPE) and DPE vapor on the cellular proteome and the ability of a known anti-oxidant Vitamin E in ameliorating such changes. LC-MS3 analysis of DPE and/or its vapor exposed skin keratinocytes resulted in quantification of 4,490 proteins of which 201 and 374 proteins were significantly dysregulated (≥1.5 fold, p-value ≥0.05) in each condition, respectively. We observed distinct molecular alterations in chronic DPE and DPE vapor exposure models. Dysregulation of several cellular processes such as cell cycle and gene regulation, oxidative stress response proteins, skin barrier integrity and skin hydration were observed in both DPE and DPE vapor exposed cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Skin

SUBMITTER: Aditi Chatterjee

LAB HEAD: Aditi Chatterjee

PROVIDER: PXD006743 | Pride | 2018-11-06

REPOSITORIES: Pride

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Publications

Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract-A role for antioxidants in skin health.

Rajagopalan Pavithra P Jain Ankit P AP Nanjappa Vishalakshi V Patel Krishna K Mangalaparthi Kiran K KK Babu Niraj N Cavusoglu Nükhet N Roy Nita N Soeur Jeremie J Breton Lionel L Pandey Akhilesh A Gowda Harsha H Chatterjee Aditi A Misra Namita N

Journal of dermatological science 20180521 3

<h4>Background</h4>Skin acts as a protective barrier against direct contact with pollutants but inhalation and systemic exposure have indirect effect on keratinocytes. Exposure to diesel exhaust has been linked to increased oxidative stress.<h4>Objective</h4>To investigate global proteomic alterations in diesel particulate extract (DPE)/its vapor exposed skin keratinocytes.<h4>Methods</h4>We employed Tandem Mass Tag (TMT)-based proteomics to study effect of DPE/DPE vapor on primary skin keratino ...[more]

PMID: 29857962

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Dataset Information

Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract – a role for antioxidants in skin health

Publications

Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract-A role for antioxidants in skin health.

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