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Comparative analysis of four- and seven-day-old Arabidopsis thaliana plasmodesmata fractions using label free method

ABSTRACT: To compare four- and seven-day-old Arabidopsis thaliana plasmodesmata proteome, the identification and the relative amount of proteins in plasmodesmata, cell wall and total cell extracts were performed using fractions purified from four- and seven-day-old liquid culture cells of A. thaliana. The relative amount for each protein was determined with a label-free quantification method. Four biological replicates of each fraction were used for quantification.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Stephane Claverol

LAB HEAD: Emmanuelle Bayer

PROVIDER: PXD006806 | Pride | 2019-07-05

REPOSITORIES: Pride

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Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.

Brault Marie L ML Petit Jules D JD Immel Françoise F Nicolas William J WJ Glavier Marie M Brocard Lysiane L Gaston Amèlia A Fouché Mathieu M Hawkins Timothy J TJ Crowet Jean-Marc JM Grison Magali S MS Germain Véronique V Rocher Marion M Kraner Max M Alva Vikram V Claverol Stéphane S Paterlini Andrea A Helariutta Ykä Y Deleu Magali M Lins Laurence L Tilsner Jens J Bayer Emmanuelle M EM

EMBO reports 20190709 8

In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)-plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of ...[more]

PMID: 31286648

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Establishment of plasmodesmata core proteome using label free semi-quantitative proteomic analysis.

Project description:To establish the plasmodesmata core proteome, the identification and the relative amount of proteins in different cellular fractions, namely, the plasmodesmata, plasma membrane, total cell extract, microsomal and cell wall fractions, were determined using label-free quantification method. Four to six biological replicates for each fraction were used for quantification.

2019-07-05 | PXD006800 | Pride

Comparative phyloproteomics identifies conserved plasmodesmal proteins

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2023-01-13 | PXD038964 | Pride

Proteomic analysis of plasmodesmata from Populus cell suspension cultures

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2018-12-31 | PXD010692 | Pride

Transcription profiling of Arabidopsis seed germination in presence and absence of histone deaceatylase inhibitor, Trichostain A (TSA).

Project description:Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at one day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes. Experiment Overall Design: Three samples: Unimbibed seeds, untreated imbibed seeds, TSA-treated imbibed seeds.

2008-06-12 | E-GEOD-3784 | biostudies-arrayexpress

Transcription profiling of Arabidopsis treated with Histone acetylation during seed germination (GEO Super Series)

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2008-06-12 | E-GEOD-3799 | biostudies-arrayexpress

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2008-06-12 | E-GEOD-3783 | biostudies-arrayexpress

Expression profiles of embryo-defective Arabidopsis thaliana mutants increased size exclusion limit1 (ise1) and ise2

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D. melanogaster gene expression profiling time course of Snail mutants

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2014-01-10 | E-MTAB-1598 | biostudies-arrayexpress

A high confidence Physcomitrium patens plasmodesmata proteome

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2024-01-18 | PXD032820 | Pride

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2023-11-27 | GSE248400 | GEO

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