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Proteogenomic analysis of Mehtylobacterium extorquens DM4

ABSTRACT: Proteogenomic analysis of Methylobacterium extorquens DM4 using the dN-TOP strategy with heavy and light TMPP labelling

INSTRUMENT(S): Q Exactive

ORGANISM(S): Methylobacterium Extorquens Dm4

SUBMITTER: Gauthier Husson

LAB HEAD: Christine CARAPITO

PROVIDER: PXD006836 | Pride | 2018-04-25

REPOSITORIES: Pride

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N-terminome and proteogenomic analysis of the Methylobacterium extorquens DM4 reference strain for dichloromethane utilization.

Bibi-Triki Sabrina S Husson Gauthier G Maucourt Bruno B Vuilleumier Stéphane S Carapito Christine C Bringel Françoise F

Journal of proteomics 20180319

Methylobacterium strains can use one-carbon compounds, such as methanol, for methylotrophic growth. In addition to methanol, a few strains also utilize dichloromethane, a major industrial chlorinated solvent pollutant. With a fully assembled and annotated genome, M. extorquens DM4 is the reference bacterium for aerobic dichloromethane degradation. The doublet N-terminal oriented proteomics (dN-TOP) strategy was applied to further improve its genome annotation and a differential proteomics approa ...[more]

PMID: 29567292

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