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Arabidopsis Myristoylom - Targeted Profiling of Arabidopsis thaliana Subproteomes Illuminates Co- and Posttranslationally N-Terminal Myristoylated Proteins

ABSTRACT: analysis of arabidopsis membrane fraction targeted towards the idenitification of myristoylated proteins

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: Wojciech Majeran

LAB HEAD: Carmela Gilgione

PROVIDER: PXD006850 | Pride | 2019-12-09

REPOSITORIES: Pride

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			Action	DRS
	111124_PML1.raw	Raw
	111124_PML1_F016598.dat	Other
	111124_PML1_F016599.dat	Other
	111124_PML1_F016600.dat	Other
	111124_PML1_F016601.dat	Other

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Targeted Profiling of <i>Arabidopsis thaliana</i> Subproteomes Illuminates Co- and Posttranslationally N-Terminal Myristoylated Proteins.

Majeran Wojciech W Le Caer Jean-Pierre JP Ponnala Lalit L Meinnel Thierry T Giglione Carmela C

The Plant cell 20180216 3

N-terminal myristoylation, a major eukaryotic protein lipid modification, is difficult to detect in vivo and challenging to predict in silico. We developed a proteomics strategy involving subfractionation of cellular membranes, combined with separation of hydrophobic peptides by mass spectrometry-coupled liquid chromatography to identify the <i>Arabidopsis thaliana</i> myristoylated proteome. This approach identified a starting pool of 8837 proteins in all analyzed cellular fractions, comprising ...[more]

PMID: 29453228

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