Proteomics

Dataset Information

32

A peptidomic approach to characterize peptides involved in cerebellar cortex development leads to the identification of the neurotrophic effects of nociceptin


ABSTRACT: The cerebellum is a brain structure involved in motor and cognitive functions. The development of the cerebellar cortex (the external part of the cerebellum) is under the control of numerous factors. Among these factors, neuropeptides including PACAP or somatostatin modulate the survival, migration and/or differentiation of cerebellar granule cells. Interestingly, such peptides contributing to cerebellar ontogenesis usually exhibit a specific transient expression profile with a low abundance at birth, a high expression level during the developmental processes, which take place within the first two postnatal weeks in rodents, and a gradual decline toward adulthood. Thus, to identify new peptides transiently expressed in the cerebellum during development, rat cerebella were sampled from birth to adulthood, and analyzed by a semi-quantitative peptidomic approach. A total of 33 peptides were found to be expressed in the cerebellum. Among these 33 peptides, 8 had a clear differential expression pattern during development, 4 of them i.e. cerebellin 2, nociceptin, somatostatin and VGF [353-372], exhibiting a high expression level during the first two postnatal weeks followed by a significative decrease at adulthood. A focus by a genomic approach on nociceptin, confirmed that the precursor mRNA is transiently expressed during the first week of life in granule neurons within the internal granule cell layer of the cerebellum, and showed that the nociceptin receptor is also actively expressed between P8 and P16 by the same neurons. Finally, functional studies revealed a new role for nociceptin, acting as a neurotrophic peptide able to promote the survival and differentiation of developing cerebellar granule neurons.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus norvegicus  

TISSUE(S): Brain

DISEASE(S): Not Available

SUBMITTER: Auriane Corbière  

LAB HEAD: Anouar Youssef

PROVIDER: PXD006874 | Pride | 2018-06-15

REPOSITORIES: Pride

altmetric image

Publications

A Peptidomic Approach to Characterize Peptides Involved in Cerebellar Cortex Development Leads to the Identification of the Neurotrophic Effects of Nociceptin.

Corbière Auriane A   Walet-Balieu Marie-Laure ML   Chan Philippe P   Basille-Dugay Magali M   Hardouin Julie J   Vaudry David D  

Molecular & cellular proteomics : MCP 20180612 9


The cerebellum is a brain structure involved in motor and cognitive functions. The development of the cerebellar cortex (the external part of the cerebellum) is under the control of numerous factors. Among these factors, neuropeptides including PACAP or somatostatin modulate the survival, migration and/or differentiation of cerebellar granule cells. Interestingly, such peptides contributing to cerebellar ontogenesis usually exhibit a specific transient expression profile with a low abundance at  ...[more]

Similar Datasets

2016-06-30 | E-GEOD-82097 | ArrayExpress
2017-05-16 | E-MTAB-5681 | ArrayExpress
2016-06-30 | E-GEOD-75696 | ArrayExpress
2018-06-25 | GSE84098 | GEO
2010-02-21 | E-GEOD-18617 | ArrayExpress
2010-02-12 | GSE18617 | GEO
2015-09-08 | E-GEOD-18617 | ExpressionAtlas
2013-09-13 | E-GEOD-50824 | ArrayExpress
| GSE108480 | GEO
2013-01-17 | E-GEOD-26750 | ArrayExpress