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Analysis of isotope incorporation in differentiated and undifferentiated podocytes

ABSTRACT: We subjected podocytes (either differentiated or undifferentiated) to a pulse with stable isotope labeled amino acids for 24, 48, 72 and 96h.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD006887 | Pride | 2018-05-01

REPOSITORIES: Pride

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Protein half-life determines expression of proteostatic networks in podocyte differentiation.

Schroeter Christina B CB Koehler Sybille S Kann Martin M Schermer Bernhard B Benzing Thomas T Brinkkoetter Paul T PT Rinschen Markus M MM

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20180425 9

Podocytes are highly specialized, epithelial, postmitotic cells, which maintain the renal filtration barrier. When adapting to considerable metabolic and mechanical stress, podocytes need to accurately maintain their proteome. Immortalized podocyte cell lines are a widely used model for studying podocyte biology in health and disease in vitro. In this study, we performed a comprehensive proteomic analysis of the cultured human podocyte proteome in both proliferative and differentiated conditions ...[more]

PMID: 29694247

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