Comparative analyses of data independent acquisition mass spectrometric approaches: DIA, WiSIM-DIA and directDIA

ABSTRACT: With a multitude of DIA approaches developed recently, many of their features are not yet well explored. In this study we make use of a biologically relevant sample, the synaptosome, as a model to compare the spectral libraries generated by (1) DDA solely on Orbitrap (OT), (2) Orbitrap for MS1 scan and ion trap for MS/MS acquisition (IT), and (3) directDIA derived from DIA data. Next, we compared the number of spectral library features recovered, and quality of quantification, by DIA and WiSIM-DIA, and suggest future improvement.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Frank Koopmans  

LAB HEAD: Ka Wan Li

PROVIDER: PXD006934 | Pride | 2017-11-20

REPOSITORIES: Pride