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Human Leukaemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives

ABSTRACT: Cryopreservation is a routinely used methodology for prolonged storage of viable cells. The use of cryo-protective agents (CPAs) such as dimethylsulfoxide (DMSO), glycerol or trehalose is paramount to reduce cellular cryo-injury but their effectiveness is still limited. The current study focuses on establishing and modulating the proteomic and the corresponding biological profiles associated with the cryo-injury of human leukaemia (HL-60) cells cryopreserved in DMSO alone or DMSO +/- novel CPAs [e.g. nigerose (Nig) or salidroside (Sal)].

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Juliana Cassoli

LAB HEAD: Daniel Martins-de-Souza

PROVIDER: PXD006998 | Pride | 2018-11-26

REPOSITORIES: Pride

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Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives.

Al-Otaibi Noha A S NAS Cassoli Juliana S JS Martins-de-Souza Daniel D Slater Nigel K H NKH Rahmoune Hassan H

GigaScience 20190301 3

<h4>Background</h4>Cryopreservation is a routinely used methodology for prolonged storage of viable cells. The use of cryo-protective agents (CPAs) such as dimethylsulfoxide (DMSO), glycerol, or trehalose is paramount to reducing cellular cryo-injury, but their effectiveness is still limited. The current study focuses on establishing and modulating the proteomic and the corresponding biological profiles associated with the cryo-injury of human leukemia (HL-60) cells cryopreserved in DMSO alone o ...[more]

PMID: 30535373

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