Proteomics

Dataset Information

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Soil metaproteomics using DNA RNA protein co-extraction method


ABSTRACT: Soil is an inherently complex matrix and as such, we believe when performing culture-independent microbial community analyses using the 'omics' suite of tools, all biomolecules investigated should be co-extracted from the same biological sample. To this end, we developed a robust, cost-effective DNA, RNA and protein co-extraction method for soil. The samples deposited here represent 3 biological replicates from one of eight soil types tested in this work.

INSTRUMENT(S): QSTAR

ORGANISM(S): Environmental Samples

SUBMITTER: Camilla Thorn  

LAB HEAD: Florence Abram

PROVIDER: PXD007077 | Pride | 2019-07-15

REPOSITORIES: Pride

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Publications

A robust, cost-effective method for DNA, RNA and protein co-extraction from soil, other complex microbiomes and pure cultures.

Thorn Camilla E CE   Bergesch Christian C   Joyce Aoife A   Sambrano Gustavo G   McDonnell Kevin K   Brennan Fiona F   Heyer Robert R   Benndorf Dirk D   Abram Florence F  

Molecular ecology resources 20190301 2


The soil microbiome is inherently complex with high biological diversity, and spatial heterogeneity typically occurring on the submillimetre scale. To study the microbial ecology of soils, and other microbiomes, biomolecules, that is, nucleic acids and proteins, must be efficiently and reliably co-recovered from the same biological samples. Commercial kits are currently available for the co-extraction of DNA, RNA and proteins but none has been developed for soil samples. We present a new protoco  ...[more]

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