An immunoproteomic approach to characterize the CAR interactome and signalosome

ABSTRACT: Adoptive transfer of chimeric antigen receptor (CAR)-T cells is expected to become the first line of treatment for multiple malignancies, following the enormous success of anti-CD19 therapies. However, their mechanism of action is not fully understood, and clear guidelines for the design of safe and efficient receptors are missing. We hereby describe a systematic analysis of the CAR “interactome” in human primary T cells, which allowed us to identify molecular traits that influence CAR-T cell efficacy. Interactome analysis was based on immunoprecipitation of CARs followed by protein identification by mass spectrometry.

OTHER RELATED OMICS DATASETS IN: GSE102823

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture, T-lymphocyte

DISEASE(S): Cancer

SUBMITTER: John Koomen  

LAB HEAD: Daniel Abate-Daga

PROVIDER: PXD007085 | Pride | 2019-11-07

REPOSITORIES: Pride