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Proteomic analysis of mousekidney cortices with and without VPS34 knockout

ABSTRACT: We analyzed the proteome of mouse with a tubule-specific VPS34 knockout and compared it to control mice.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD007092 | Pride | 2024-01-26

REPOSITORIES: Pride

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VPS34-dependent control of apical membrane function of proximal tubule cells and nutrient recovery by the kidney.

Rinschen Markus M MM Harder Jennifer L JL Carter-Timofte Madalina E ME Zanon Rodriguez Luis L Mirabelli Carmen C Demir Fatih F Kurmasheva Naziia N Ramakrishnan Suresh K SK Kunke Madlen M Tan Yifan Y Billing Anja A Dahlke Eileen E Larionov Alexey A AA Bechtel-Walz Wibke W Aukschun Ute U Grabbe Marlen M Nielsen Rikke R Christensen Erik I EI Kretzler Matthias M Huber Tobias B TB Wobus Christiane E CE Olagnier David D Siuzdak Gary G Grahammer Florian F Theilig Franziska F

Science signaling 20221129 762

The lipid kinase VPS34 orchestrates autophagy, endocytosis, and metabolism and is implicated in cancer and metabolic disease. The proximal tubule in the kidney is a key metabolic organ that controls reabsorption of nutrients such as fatty acids, amino acids, sugars, and proteins. Here, by combining metabolomics, proteomics, and phosphoproteomics analyses with functional and superresolution imaging assays of mice with an inducible deficiency in proximal tubular cells, we revealed that VPS34 contr ...[more]

PMID: 36445937

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