Browse
Submit Data
Databases
API
Help

Dataset Information

183 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of thermal stress on Lactobacillus casei GCRL163

ABSTRACT: Identification of biomarkers and mechanisms of adaptation to prolonged thermal stress in L casei strain GCRL163

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Richard Wilson

LAB HEAD: Dr Richard Wilson

PROVIDER: PXD007097 | Pride | 2018-11-04

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Proteomic analysis of Lactobacillus casei GCRL163 cell-free extracts reveals a SecB homolog and other biomarkers of prolonged heat stress.

Adu Kayode T KT Wilson Richard R Nichols David S DS Baker Anthony L AL Bowman John P JP Britz Margaret L ML

PloS one 20181025 10

Prolonged heat stress is one of the harsh conditions Lactobacillus casei strains encounter as non-starter lactic acid bacteria in dairy product manufacture. To understand the physiological and molecular mechanisms through which Lb. casei GCRL163 adapts to persistent elevated temperature, label-free quantitative proteomics of cell-free extracts was used to characterize the global responses of the strain cultured anaerobically in bioreactors at 30 to 45°C, pH 6.5, together with GC-MS for fatty aci ...[more]

PMID: 30359441

Similar Datasets

Surface proteome of Lactobacillus casei GCRL163 under heat adaptation

Project description:Cell surface proteins are constatntly impacted by environmental stresses. To understand the imapct of heat adaptation on the Lactobacillus casei GCRL163, trypsin shaving technique was used to extract the surface proteins of the strain and the protein fraction obtained analysed using gel-free proteomics.

2022-03-01 | PXD009468 | Pride

Surface proteome of Lactobacillus casei GCRL163 under heat adaptation – LiCl Sucrose

Project description:Cell surface proteins are constantly impacted by environmental stresses. To understand the impact of heat adaptation on the Lactobacillus casei GCRL163, trypsin shaving technique was used to extract the surface proteins of the strain and the protein fraction obtained analysed using gel-free proteomics.

2022-03-01 | PXD009591 | Pride

Surface proteome of Lactobacillus casei GCRL163 under heat adaptation – extracellular fluid

2022-03-01 | PXD009638 | Pride

Response to bile of Lactobacillus casei BL23

Project description:The response to bile of Lactobacillus casei BL23 was investigated. Lactobacillus casei BL23 was grown in MRS medium at 37C without shaking until O.D. 595 nm. reached 0.5. The cultures were then two fold diluted with prewarmed MRS medium (control) or MRS supplemented with 0.2% bovine bile (treatment) and incubation continued for 45 min. Cells were then harvested and total RNA purified.

2011-10-19 | E-GEOD-33047 | biostudies-arrayexpress

Comparative genome hybridization for Lactobacillus casei

Project description:Lactobacillus casei is remarkably adaptive to diverse habitats. To understand the evolution and adaptation of Lb. casei strains isolated from different environments, the gene content of 22 Lb. casei strains isolated from various habitats (cheeses, n=8; plant materials, n=8; and human sources, n=6) were examined by comparative genome hybridization with an Lb. casei ATCC 334-based microarray. Comparative genome hybridization was performed against an Affymetrix custom microarray designed to include 2,661 (97%) chromosomal and 17 (85%) plasmid CDSs predicted to occur in Lb. casei ATCC 334, as well as all predicted CDSs in the draft Lb. helveticus CNRZ 32 genome. CDSs that were not included in the microarray design were all transposase-encoding genes.

2010-06-21 | E-GEOD-15030 | biostudies-arrayexpress

Comparison of the response to bile of Lactobacillus casei BL23 and its derivative strain TC01

Project description:The response to bile of Lactobacillus casei BL23 and its derivative strain TC01 was investigated. TC01 strain carries a complete deletion of gene LCABL_02080 which encodes a two component signal transduction response regulator. Lactobacillus casei BL23 and TC01 strains were grown in MRS medium at 37C without shaking until O.D. 595 nm. reached 0.5. The cultures were then two fold diluted with prewarmed MRS medium (control) or MRS supplemented with 0.2% bovine bile (treatment) and incubation continued for 45 min. Cells were then harvested and total RNA purified.

2012-10-10 | E-GEOD-25448 | biostudies-arrayexpress

Gene expression profile of Lactobacillus casei Zhang in fermented milk during transit in simulated gastrointestinal tract

Project description:The survival of probiotics could be strongly enhanced by delivery vehicles, but the mechanism was unknown. In this study, whole genome microarray technology was used to detect the gene expression profiles of probiotic Lactobacillus casei Zhang in the absence and presence of fermented milk in simulated gastrointestinal tract. The results showed the gene expression profiles were significantly different under these two different conditions. The change of the gene expression profile may be helpful to comprehend the role of delivery vehicles in enhancing the survival of probiotics. Twelve samples of bacterial cells in the absence and presence of fermented milk were collected at 3 h in simulated gastric juice, 4 h and 8 h in intestinal juice, 2 biological replicates were obtained in each time point. The average of the normalized expression values of the 2 biological replicates for each probe was regarded as the expression value of a predicted gene. To identify the expression pattern of each gene across different treatments, n-fold change ratios were calculated for every gene in pure L. casei Zhang versus L. casei Zhang in fermented milk.

2011-12-31 | E-GEOD-23685 | biostudies-arrayexpress

Comparative genome hybridization for Lactobacillus casei

2009-03-17 | GSE15030 | GEO

Response to bile of Lactobacillus casei BL23

Project description:The response to bile of Lactobacillus casei BL23 was investigated.

2011-10-20 | GSE33047 | GEO

Integrative genomic and proteomic analysis of response of Lactobacillus casei str. Zhang to glucose limitation

Project description:Nutrient starvation is an important survival challenge for bacteria during industrial production of functional foods. Lactobacilli are increasingly being used as probiotics in functional foods. As next-generation sequencing technology has greatly advanced, we performed integrative proteomic and genomic analysis to investigate the response of Lactobacillus casei Zhang to a glucose-restricted environment. L. casei Zhang strains were permitted to evolve in glucose-limited or normal medium from a common ancestor over a 3-year period, and they were sampled after 1000, 2000, 3000, 4000, 5000, 6000, 7000, and 8000 generations and subjected to proteomic and genomic analyses. Genomic resequencing data revealed different point mutations and other mutational events in each generation of L. casei Zhang under glucose limitation stress. The proteins expressed differentially under glucose limitation were found to be significantly related to fructose and mannose metabolism, carbohydrate metabolic processes, lyase activity, and amino acid-transporting ATPase activity. The integrative proteomic and genomic analysis revealed that the mutations protected L. casei Zhang against glucose starvation by regulating other cellular carbohydrate, fatty acid, and amino acid catabolism; phosphoenolpyruvate system pathway activation; glycogen synthesis; ATP consumption; pyruvate metabolism; and general stress response protein expression. The results help reveal the mechanisms of adapting to glucose starvation and provide new strategies for enhancing the industrial utility of L. casei Zhang.

2018-02-08 | PXD006643 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data