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Phosphoproteomic analysis of the X-ray induced DNA damage response in dividing cells from wild type and atm mutants using LC-MSMS

ABSTRACT: Rapid and effective DNA damage responses are required for plant growth and maintenance of active meristems under conditions of genotoxic stress. Here, analysis of the post-translational signalling network in mitotically dividing cells revealed a wide network of highly dynamic changes in the phosphoprotein profile of genotoxin treated cells, mediated in part by the ATAXIA TELANGIECTASIA MUTATED (ATM) protein kinase

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Primary Cell

SUBMITTER: Chris West

LAB HEAD: Chris West

PROVIDER: PXD007100 | Pride | 2019-08-19

REPOSITORIES: Pride

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Phosphoproteomic analysis reveals plant DNA damage signalling pathways with a functional role for histone H2AX phosphorylation in plant growth under genotoxic stress.

Waterworth Wanda M WM Wilson Michael M Wang Dapeng D Nuhse Thomas T Warward Stacey S Selley Julian J West Christopher E CE

The Plant journal : for cell and molecular biology 20190910 5

DNA damage responses are crucial for plant growth under genotoxic stress. Accumulating evidence indicates that DNA damage responses differ between plant cell types. Here, quantitative shotgun phosphoproteomics provided high-throughput analysis of the DNA damage response network in callus cells. MS analysis revealed a wide network of highly dynamic changes in the phosphoprotein profile of genotoxin-treated cells, largely mediated by the ATAXIA TELANGIECTASIA MUTATED (ATM) protein kinase, represen ...[more]

PMID: 31410901

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