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N-linked glycosylation of recombinant Porcine Delta Coronavirus spike protein ectodomain from drosophila S2 cells

ABSTRACT: Recombinant PDCoV spike glycoprotein was produced from Drosophile S2 cells for structural characterization by cryo electron microscopy and mass spectrometry. MS was used to assess the N-linked glycosylation profile of the purified material. We used a multiple protease digestion in combination with RP-LC-MS/MS, EThcD fragmentation on Orbitrap Fusion to characterize intact glycopeptides.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Deltacoronavirus Pdcov/usa/illinois121/2014

SUBMITTER: Joost Snijder

LAB HEAD: David Veesler

PROVIDER: PXD007107 | Pride | 2017-10-24

REPOSITORIES: Pride

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