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Proteomic analysis of [PSI+] yeast strain

ABSTRACT: The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI+] prion state leads to more translational read-through. Although extensive studies have been done on [PSI+], changes at the proteomic level have not been performed exhaustively. We therefore used a SILAC-based quantitative mass spectrometry approach and identified 4187 proteins from both [psi-] and [PSI+] strains. Surprisingly, there was very little difference between the two proteomes under standard growth conditions.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Haploid

DISEASE(S): Disease Free

SUBMITTER: Patrick Hau Wing Chan

LAB HEAD: Thibault Mayor

PROVIDER: PXD007168 | Pride | 2018-10-24

REPOSITORIES: Pride

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The [PSI +] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI +] cells can promote aneuploidy.

Chan Patrick H W PHW Lee Lisa L Kim Erin E Hui Tony T Stoynov Nikolay N Nassar Roy R Moksa Michelle M Cameron Dale M DM Hirst Martin M Gsponer Joerg J Mayor Thibault T

Scientific reports 20170816 1

The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI +] prion state leads to more translational read-through. Although extensive studies have been done on [PSI +], changes at the proteomic level have not been performed exhaustively. We therefore used a SILAC-based quantitative mass spectrometry approach and identified 4187 proteins from both [psi -] and [PSI +] strains. Surprisingly, there was very ...[more]

PMID: 28814753

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