Proteomics

Dataset Information

32

Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves


ABSTRACT: Protein lysine acylation, such as succinylation and acetylation are important posttranslational modification (PTM) mechanisms, with key roles in the regulation of diverse metabolic and cellular processes. Antibody-based affinity enrichment, high-resolution LC-MS/MS analysis and integrated bioinformatics analysis, were used to characterize the lysine succinylome (Ksuc) and acetylome (Kace) in rice leaves. Rice (Oryza sativa., Wuyunjing 7) was kindly provided byJiangsu Academy of Agricultural Sciences, Jiangsu Province, China. Rice seeds were surface-sterilized with 5% NaClO for 20 min, washed extensively with distilled water and then germinated in distilled water at 28ºC for 2 days. Germinated seeds were cultivated on half-strength ammonium-free Murashige and Skoog (MS) medium. For oxidative stress treatments, 14-days-old rice seedlings were transferred to 1/2 MS medium in the absence or presence of 1 mM H2O2 for 6 days. Rice seedlings were then harvested for protein extraction.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza sativa  

TISSUE(S): Leaf

DISEASE(S): Not Available

SUBMITTER: Iris Finkemeier  

LAB HEAD: Prof. Iris Finkemeier

PROVIDER: PXD007175 | Pride | 2018-07-23

REPOSITORIES: pride

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Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves.

Zhou Heng H   Finkemeier Iris I   Guan Wenxue W   Tossounian Maria-Armineh MA   Wei Bo B   Young David D   Huang Jingjing J   Messens Joris J   Yang Xibin X   Zhu Jun J   Wilson Michael H MH   Shen Wenbiao W   Xie Yanjie Y   Foyer Christine H CH  

Plant, cell & environment 20180109 5


Protein lysine acylations, such as succinylation and acetylation, are important post-translational modification (PTM) mechanisms, with key roles in cellular regulation. Antibody-based affinity enrichment, high-resolution liquid chromatography mass spectrometry analysis, and integrated bioinformatics analysis were used to characterize the lysine succinylome (Ksuc ) and acetylome (Kace ) of rice leaves. In total, 2,593 succinylated and 1,024 acetylated proteins were identified, of which 723 were s  ...[more]

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