Dataset Information

Proteomic analysis of L6 myoblasts treated with C-terminal transgelin peptides

ABSTRACT: To investigate bioactivity of peptides identified in human plasma, we treated L6 myoblasts in vitro with a synthetic C-terminal peptide from TAGLN spanning amino acids 179-200, or a synthetic C-terminal peptide from TAGLN2 spanning amino acids 178-199 (1 µg/mL; 7 days), and compared the treatments to control myoblasts cultured for the same amount of time with the addition of only PBS. Proteomic analysis by single-shot nanoUHPLC-MS/MS was performed on four biological replicates and quantification performed by LFQ. A total of 6,095 proteins were identified with 4,710 quantified in at least three biological replicates (Supplemental Table S4). A total of 222 and 307 proteins were regulated by the TAGLN and TAGLN2 peptides relative to the control treatments, respectively, with 61 proteins co-regulated by both TAGLN and TAGLN2 (P<0.05; two-sample t-test with Benjamini-Hochberg correction) (Figure 3G).

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Myoblast

SUBMITTER: Benjamin Parker

LAB HEAD: Benjamin Parker

PROVIDER: PXD007176 | Pride | 2017-11-21

REPOSITORIES: Pride

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			Action	DRS
	170107_BenP_QEHF_Ctrl_1.raw	Raw
	170107_BenP_QEHF_Ctrl_2.raw	Raw
	170107_BenP_QEHF_Ctrl_3.raw	Raw
	170107_BenP_QEHF_Ctrl_4.raw	Raw
	170107_BenP_QEHF_T1_1.raw	Raw

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Publications

Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome.

Parker Benjamin L BL Burchfield James G JG Clayton Daniel D Geddes Thomas A TA Payne Richard J RJ Kiens Bente B Wojtaszewski Jørgen F P JFP Richter Erik A EA James David E DE

Molecular & cellular proteomics : MCP 20171005 12

Exercise is extremely beneficial to whole body health reducing the risk of a number of chronic human diseases. Some of these physiological benefits appear to be mediated via the secretion of peptide/protein hormones into the blood stream. The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and PTMs, and is a rich source of hormones. In the current study we have quantified the effects of intense exercise on the ...[more]

PMID: 28982716

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