Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein

ABSTRACT: Mapping the interaction sites between membrane spanning proteins is a key challenge in structural biology. Here we develop and apply carbene footprinting technology to identify the interfacial sites of a trimeric membrane protein. We show how the footprinting probe is effectively incorporated into detergent micelles leading to efficient labelling of the external membrane-spanning regions of the protein.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Lucio Manzi  

LAB HEAD: Neil J. Oldham

PROVIDER: PXD007207 | Pride | 2017-09-29

REPOSITORIES: Pride