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Proteome of ATGL knock-out A549 cells compared to A549 wild type cells

ABSTRACT: Comparison of ATGL-KO and ATGL-WT A549 cell proteomes

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

DISEASE(S): Lung Cancer

SUBMITTER: Barbara Darnhofer

LAB HEAD: Ruth Birner-Gruenberger

PROVIDER: PXD007223 | Pride | 2018-09-18

REPOSITORIES: Pride

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Deletion of Adipose Triglyceride Lipase Links Triacylglycerol Accumulation to a More-Aggressive Phenotype in A549 Lung Carcinoma Cells.

Tomin Tamara T Fritz Katarina K Gindlhuber Juergen J Waldherr Linda L Pucher Bettina B Thallinger Gerhard G GG Nomura Daniel K DK Schittmayer Matthias M Birner-Gruenberger Ruth R

Journal of proteome research 20180307 4

Adipose triglyceride lipase (ATGL) catalyzes the rate limiting step in triacylglycerol breakdown in adipocytes but is expressed in most tissues. The enzyme was shown to be lost in many human tumors, and its loss may play a role in early stages of cancer development. Here, we report that loss of ATGL supports a more-aggressive cancer phenotype in a model system in which ATGL was deleted in A549 lung cancer cells by CRISPR/Cas9. We observed that loss of ATGL led to triacylglycerol accumulation in ...[more]

PMID: 29457907

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