Proteomics

Dataset Information

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Human melanoma cell lines secretome analysis


ABSTRACT: The imbalance of cellular homeostasis during oncogenesis together with the high heterogeneity of tumor-associated stromal cells have a marked effect on the repertoire of the proteins secreted by malignant cells (the secretome). Hence, the study of tumoral secretomes provides insights for understanding the cross-talk between cells within the tumor microenvironment as well as the key effectors for the establishment of the pre-metastatic niche in distant tumor sites. In this context, we performed a proteomic analysis of the secretomes derived from four cell lines: (i) a paired set of fibroblasts - Hs 895. T, a cell line obtained from a lung node metastatic site from a patient who had melanoma and Hs 895.Sk, a skin fibroblast cell line (derived from the same patient); (ii) two malignant metastatic melanoma cell lines - A375, a malignant melanoma cell line from primary source and SH-4, a cell line derived from pleural effusion of a patient with metastatic melanoma. Clustering of expression profiles together with functional enrichment revealed patterns that mirrored each cell type (skin fibroblasts, cancer-associated fibroblasts and metastatic cells). These patterns might be the result of cell-specific protein expression programs and may serve as basis for further proteomic analysis of melanoma cell lines secretomes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Cell Culture, Fibroblast

DISEASE(S): Melanoma

SUBMITTER: Andre Zelanis  

LAB HEAD: André Zelanis

PROVIDER: PXD007265 | Pride | 2018-01-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OB_AZP_1_20160827_01.raw Raw
OB_AZP_2_20160827_02.raw Raw
OB_AZP_3_20160827_03.raw Raw
OB_AZP_4_20160827_04_160905133413.raw Raw
OB_AZP_5_20160827_05_160905163243.raw Raw
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Publications

Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines.

Liberato Tarcísio T   Pessotti Dayelle S DS   Fukushima Isabella I   Kitano Eduardo S ES   Serrano Solange M T SMT   Zelanis André A  

Journal of proteomics 20171222


The imbalance of cellular homeostasis during oncogenesis together with the high heterogeneity of tumor-associated stromal cells have a marked effect on the repertoire of the proteins secreted by malignant cells (the secretome). Hence, the study of tumoral secretomes provides insights for understanding the cross-talk between cells within the tumor microenvironment as well as the key effectors for the establishment of the pre-metastatic niche in distant tumor sites. In this context, we performed a  ...[more]

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