UPLC-MS/MS of YAP co-IP during activating and inactivating plating conditions
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Mammary Epithelial Cell Line
SUBMITTER: 
Ryan Leib
LAB HEAD: Ovijit Chaudhuri
PROVIDER: PXD007287 | Pride | 2019-11-21
REPOSITORIES: Pride
Publications
Identification of cell context-dependent YAP-associated proteins reveals β<sub>1</sub> and β<sub>4</sub> integrin mediate YAP translocation independently of cell spreading.
Lee Joanna Y JY Dominguez Antonia A AA Nam Sungmin S Stowers Ryan S RS Qi Lei S LS Chaudhuri Ovijit O
Scientific reports 20191120 1
Yes-associated protein (YAP) is a transcriptional regulator and mechanotransducer, relaying extracellular matrix (ECM) stiffness into proliferative gene expression in 2D culture. Previous studies show that YAP activation is dependent on F-actin stress fiber mediated nuclear pore opening, however the protein mediators of YAP translocation remain unclear. Here, we show that YAP co-localizes with F-actin during activating conditions, such as sparse plating and culturing on stiff 2D substrates. To i ...[more]
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