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Pitcher fluid protein profiling from Nepenthes spp.

ABSTRACT: Pitcher fluids from 3 species of Nepenthes, namely N. ampullaria, N. rafflesiana and their hybrid H. x hookeriana were collected within 24h of opening, filtered, concentrated, processed and trypsin-digested for mass spectrometry analysis using Thermo Easy-nLC Orbitrap Fusion Tribid MS. Analysis was performed in 2 biological replicates of pools of 9 pitchers for each species and analysed in 3 technical replicates each.

OTHER RELATED OMICS DATASETS IN: PRJNA299862

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Nepenthes Rafflesiana Nepenthes X Hookeriana Nepenthes Ampullaria

TISSUE(S): Pitcher Secretion

SUBMITTER: Hoe-Han Goh

LAB HEAD: HOE-HAN GOH

PROVIDER: PXD007599 | Pride | 2018-11-29

REPOSITORIES: Pride

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Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants.

Zulkapli Muhammad Mu'izzuddin MM Rosli Muhammad Aqil Fitri MAF Salleh Faris Imadi Mohd FIM Mohd Noor Normah N Aizat Wan Mohd WM Goh Hoe-Han HH

Genomics data 20170503

Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule rea ...[more]

PMID: 28529881

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