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Pseudomonas aeruginosa BONCAT biofilms - rpoS vs. trc

ABSTRACT: Cell-selective BONCAT analysis of proteins synthesized by two P. aeruginosa biofilm subpopulations.

INSTRUMENT(S): LCQ Classic

ORGANISM(S): Pseudomonas Aeruginosa (strain Ucbpp-pa14)

SUBMITTER: Brett Babin

LAB HEAD: David A. Tirrell

PROVIDER: PXD007622 | Pride | 2017-10-24

REPOSITORIES: Pride

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Selective Proteomic Analysis of Antibiotic-Tolerant Cellular Subpopulations in <i>Pseudomonas aeruginosa</i> Biofilms.

Babin Brett M BM Atangcho Lydia L van Eldijk Mark B MB Sweredoski Michael J MJ Moradian Annie A Hess Sonja S Tolker-Nielsen Tim T Newman Dianne K DK Tirrell David A DA

mBio 20171024 5

Biofilm infections exhibit high tolerance against antibiotic treatment. The study of biofilms is complicated by phenotypic heterogeneity; biofilm subpopulations differ in their metabolic activities and their responses to antibiotics. Here, we describe the use of the bio-orthogonal noncanonical amino acid tagging (BONCAT) method to enable selective proteomic analysis of a <i>Pseudomonas aeruginosa</i> biofilm subpopulation. Through controlled expression of a mutant methionyl-tRNA synthetase, we t ...[more]

PMID: 29066549

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