Dataset Information

The Adult Aedes aegypti Midgut Peritrophic Matrix Proteome

ABSTRACT: This study was undertaken to characterize the proteome of the midgut peritrophic matrix of Aedes aegypti.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Aedes Aegypti Aegypti

TISSUE(S): Peritrophic Membrane

SUBMITTER: Keith Ray

LAB HEAD: Zach N. Adelman

PROVIDER: PXD007627 | Pride | 2018-04-05

REPOSITORIES: Pride

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Publications

Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS.

Whiten Shavonn R SR Ray W Keith WK Helm Richard F RF Adelman Zach N ZN

PloS one 20180323 3

The Aedes aegypti mosquito is the principal vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus. These arboviruses are transmitted during adult female mosquito bloodfeeding. While these viruses must transverse the midgut to replicate, the blood meal must also reach the midgut to be digested, absorbed, or excreted, as aggregation of blood meal metabolites can be toxic to the female mosquito midgut. The midgut peritrophic matrix (PM), a semipermeable extracellular layer ...[more]

PMID: 29570734

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Project description:Background: Mosquito midgut is an important target for the host parasite interaction studies as this major immune organ plays a role in parasite growth, maturation and vector competence. Most important rural malaria vector of India is Proteomic approaches coupled with bioinformatics analysis have been used to study expression of functional proteins/enzymes of An. culicifacies susceptible and refractory species midgut in order to understand the mechanism of refractoriness that may help in contributing to unravel the host pathogen interactions Methodology/Principle findings: Basic shot gun proteomics approaches along with iTRAQ based differential studies followed by LC/MS/MS were employed to study the proteome of sugar fed An. culicifacies midgut. Further bioinformatics analysis was carried out using Gene ontology, SMART analysis, CELLO algorithm etc. Here proteins identified with iTRAQ Ratio more than 1.5 was considered up regulated and less than 0.67 were considered down regulated. Ratio from 1.5 – 0.67 were considered as moderate to no change. Using these criteria we observed total 80 proteins in An. culicifacies midgut and when compared susceptible species against refractory species, total 30 proteins were found to be downregulated. 3 proteins were found to be upregulated in refractory species Conclusion/Significance: Based on the present data a significant increase in number of proteins in midgut of refractory An. culicifacies species B were found that may conclude that these proteins may be responsible for the inhibiting parasite growth and linked to the melanization of oocysts or parasite lysis mechanisms in natural populations of refractory mosquito. Hence the progress of our studies at protein level suggests that these identified annotated putative proteins/enzymes may help to explore natural vector-parasite systems and reveal valuable insights into the mechanism of refractoriness which in turn further useful for bringing of novel strategies for control of malaria.

Dataset Information

The Adult Aedes aegypti Midgut Peritrophic Matrix Proteome

Publications

Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS.

Similar Datasets

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