Proteomics

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Molecular alterations elicited by chronic exposure to cigarette smoke in oral keratinocytes


ABSTRACT: Smoking is the leading cause of preventable death worldwide. It increases the risk for various diseases including respiratory diseases, vascular diseases and different cancers including lung, oral and bladder cancer. Despite being the leading cause of oral cancer, the molecular mechanisms resulting in malignancy upon cigarette smoke exposure are yet to be fully elucidated. It is crucial to note that disease development is observed upon chronic exposure to cigarette smoke, as opposed to a short-term exposure. Hence, we sought to investigate the effect of chronic smoke exposure on normal oral keratinocytes (OKF6/TERT1). We employed tandem mass tag-based quantitative proteomic and phosphoproteomic approaches to investigate the proteomic and signaling changes in OKF6/TERT1 cells chronically exposed to cigarette smoke compared to untreated cells. LC/MS2 analysis resulted in the quantification of 5,067 proteins among which expression of 360 proteins were found to be dysregulated in at least one replicate. Phosphoproteomic analysis revealed quantification of 3,647 phosphopeptides corresponding to 1,801 proteins. Majority of the dysregulated proteins were seen to be involved in cellular processes such as cell growth, cellular communication and energy metabolism. This study will aid in elucidating the effects of smoking in oral cancer and in the identification of potential candidates molecules which could serve as early detection biomarkers or therapeutic targets.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Oral Epithelium

SUBMITTER: Aditi Chatterjee  

LAB HEAD: Aditi Chatterjee

PROVIDER: PXD007637 | Pride | 2018-03-05

REPOSITORIES: Pride

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Publications

Role of protein kinase N2 (PKN2) in cigarette smoke-mediated oncogenic transformation of oral cells.

Rajagopalan Pavithra P   Nanjappa Vishalakshi V   Patel Krishna K   Jain Ankit P AP   Mangalaparthi Kiran K KK   Patil Arun H AH   Nair Bipin B   Mathur Premendu P PP   Keshava Prasad T S TS   Califano Joseph A JA   Sidransky David D   Gowda Harsha H   Chatterjee Aditi A  

Journal of cell communication and signaling 20180226 4


Smoking is the leading cause of preventable death worldwide. Though cigarette smoke is an established cause of head and neck cancer (including oral cancer), molecular alterations associated with chronic cigarette smoke exposure are poorly studied. To understand the signaling alterations induced by chronic exposure to cigarette smoke, we developed a cell line model by exposing normal oral keratinocytes to cigarette smoke for a period of 12 months. Chronic exposure to cigarette smoke resulted in i  ...[more]

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