Proteomics

Dataset Information

69

Argentilactone molecular targets in Paracoccidioides brasiliensis identified by chemoproteomics


ABSTRACT: The etiological agents of PCM belong to the Paracoccidioides genus, which is restricted to Latin America. The infection is acquired through inhalation of conidia that primarily lodge in the lungs and may disseminate to other organs/tissues. Treatment of PCM is commonly achieved via administration of antifungals of the azole class, sulfonamides, and amphotericin B. The antifungal toxicity and side effects, added to the long treatment time has driven research for new bioactive compounds. Argentilactone, compound isolated from a Brazilian savanna plant Hyptis ovaliofolia, has been suggested as potent antifungal, inhibiting dimorphism of P. brasiliensis and the enzymatic activity of isocitrate lyase, a key enzyme of the glyoxylate cycle. Furthermore, it has no cytotoxicity and genotoxicity in fibroblast cells at concentrations that inhibit the fungal growth. This work was developed due to the importance of elucidating the putative mode of action of argentilactone. The chemoproteomics approach, by affinity chromatography, was the methodology used to explore the interactions between P. brasiliensis proteins and argentilactone

INSTRUMENT(S): nanoACQUITY UPLC

ORGANISM(S): Paracoccidioides brasiliensis Pb18  

TISSUE(S): Cell Culture

DISEASE(S): Paracoccidioidomycosis

SUBMITTER: Lívia Do Carmo Silva  

LAB HEAD: Lívia do Carmo Silva

PROVIDER: PXD007680 | Pride | 2018-02-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Pb18_arg_cito_R001.rar Other
Pb18_arg_cito_R001_XML.rar Other
Pb18_arg_cito_R002.rar Other
Pb18_arg_cito_R002_XML.rar Other
Pb18_arg_cito_R003.rar Other
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Publications

Argentilactone Molecular Targets in Paracoccidioides brasiliensis Identified by Chemoproteomics.

Silva Lívia do Carmo LDC   Tauhata Sinji Borges Ferreira SBF   Baeza Lilian Cristiane LC   de Oliveira Cecília Maria Alves CMA   Kato Lucília L   Borges Clayton Luiz CL   de Almeida Soares Célia Maria CM   Pereira Maristela M  

Antimicrobial agents and chemotherapy 20181024 11


Paracoccidioidomycosis (PCM) is the cause of many deaths from systemic mycoses. The etiological agents of PCM belong to the Paracoccidioides genus, which is restricted to Latin America. The infection is acquired through the inhalation of conidia that primarily lodge in the lungs and may disseminate to other organs and tissues. The treatment for PCM is commonly performed via the administration of antifungals such as amphotericin B, co-trimoxazole, and itraconazole. The antifungal toxicity and sid  ...[more]

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