Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive Plus, LTQ Orbitrap Elite
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Cerebellum, Glial Cell, Brain, Purkinje Cell, Hippocampal Neuron, Hippocampal Pyramidal Neuron
SUBMITTER: Julian Langer
LAB HEAD: Julian David Langer
PROVIDER: PXD007703 | Pride | 2017-11-10
REPOSITORIES: Pride
Alvarez-Castelao Beatriz B Schanzenbächer Christoph T CT Hanus Cyril C Glock Caspar C Tom Dieck Susanne S Dörrbaum Aline R AR Bartnik Ina I Nassim-Assir Belquis B Ciirdaeva Elena E Mueller Anke A Dieterich Daniela C DC Tirrell David A DA Langer Julian D JD Schuman Erin M EM
Nature biotechnology 20171106 12
Although advances in protein labeling methods have made it possible to measure the proteome of mixed cell populations, it has not been possible to isolate cell-type-specific proteomes in vivo. This is because the existing methods for metabolic protein labeling in vivo access all cell types. We report the development of a transgenic mouse line where Cre-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell-type-specific labeling of nascent proteins with a n ...[more]