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Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations

ABSTRACT: High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA, Nitrosopumilus maritimus SCM1, to ammonia starvation, Cu limitation, and Cu excess. Most predicted protein-coding genes were transcribed in exponentially growing cells, and of ~74% detected in the proteome, ~6% were modified by N-terminal acetylation. The general response to ammonia starvation and Cu-stress was down-regulation of genes for energy generation and biosynthesis. Cells rapidly depleted transcripts for the A and B subunits of ammonia monooxygenase (AMO) in response to ammonia starvation, yet retained relatively high levels of transcripts for the C subunit. Thus, similar to ammonia-oxidizing bacteria, selective retention of amoC transcripts during starvation appears important for subsequent recovery, and also suggests that AMO subunit transcript ratios could be used to assess the physiological status of marine populations. Unexpectedly, cobalamin biosynthesis was upregulated in response to both ammonia starvation and Cu-stress, indicating the importance of this cofactor in retaining functional integrity during times of stress.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Nitrosopumilus Maritimus Scm1

TISSUE(S): Cell Culture

SUBMITTER: Rachel Lundeen

LAB HEAD: Anitra Ingalls

PROVIDER: PXD007728 | Pride | 2017-10-27

REPOSITORIES: Pride

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Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations.

Qin Wei W Amin Shady A SA Lundeen Rachel A RA Heal Katherine R KR Martens-Habbena Willm W Turkarslan Serdar S Urakawa Hidetoshi H Costa Kyle C KC Hendrickson Erik L EL Wang Tony T Beck David Ac DA Tiquia-Arashiro Sonia M SM Taub Fred F Holmes Andrew D AD Vajrala Neeraja N Berube Paul M PM Lowe Todd M TM Moffett James W JW Devol Allan H AH Baliga Nitin S NS Arp Daniel J DJ Sayavedra-Soto Luis A LA Hackett Murray M Armbrust E Virginia EV Ingalls Anitra E AE Stahl David A DA

The ISME journal 20171020 2

High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA, Nitrosopumilus maritimus SCM1, to ammonia starvation, Cu limitation and Cu excess. Most predicted protein-coding genes were trans ...[more]

PMID: 29053148

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Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations

Publications

Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations.

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