Browse
Submit Data
Databases
API
Help

Dataset Information

365 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human keratinocytes HaCaT LC-MS/MS

ABSTRACT: Triton X-100 has been used as a model substance to study the effects of irritants on the skin. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of Triton X-100, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to Triton X-100 was analyzed by LC-MS/MS approach and quantified using Progenesis LC software.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Keratinocyte

SUBMITTER: Olesya Larina

LAB HEAD: Lisitsa AV

PROVIDER: PXD007776 | Pride | 2017-11-03

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

[Proteomic profiling of HaCaT keratinocytes exposed to skin damaging detergents].

Rusanov A L AL Petushkova N A NA Poverennaya E V EV Nakhod K V KV Larina O V OV Lisitsa A V AV Luzgina N G NG

Biomeditsinskaia khimiia 20171001 5

The effects of sodium dodecyl sulfate (25 mg/ml) and Triton X-100 (12.5 mg/ml and 25 mg/ml) on the HaCaT immortalized keratinocytes exposed to these surfactants for 48 h were studied. Using shotgun proteomics, a comparative analysis of the proteomic profiles of control and experimental cells after surfactants exposure was carried out. 260 common proteins were identified in control and experimental cells; 33 proteins were found in cells exposed to all three treatments, but not in control cells. T ...[more]

PMID: 29080872

Similar Datasets

Human keratinocytes HaCaT LC-MSMS_SDS exposure

Project description:We employed human HaCaT cells as a model system to identify cellular proteins that accompany SDS-induced toxicity based on a proteomic approach. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of SDS (25 µg/ml, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to SDS was analyzed by LC-MS/MS approach and quantified using Progenesis LC software. The abundance of 217 proteins (which were identified by multiple peptides, ≥ 2) was altered in keratinocytes exposed to SDS; in which 131 proteins had increased abundance while 86 proteins was down regulated. The Pathview map of 131 up-regulated proteins was built and enhancement of glycolysis/gluconeogenesis was found.

2017-11-03 | PXD007789 | Pride

Temporal gene regulation of human keratinocyte HaCaT cells treated with obacunone

Project description:Obacunone treatment upregulates gene expression of cholesterol metabolism-associated genes in HaCaT cells Total RNA obtained from HaCaT cells treated with 100 uM obacunone for 0.5, 1, 3, 6, or 12 h was compared to that of vehicle control cells.

2014-04-29 | E-GEOD-57080 | biostudies-arrayexpress

Gene expression profile analysis of human keratinocyte HaCaT cells treated with obacunone

Project description:Obacunone treatment upregulates gene expression of cholesterol and lipid metabolism-associated genes in HaCaT cells Total RNA obtained from HaCaT cells treated with 10, 50, or 100 uM obacunoe for 4 h was compared to that of vehicle control cells.

2014-04-29 | E-GEOD-57120 | biostudies-arrayexpress

Triton X-100 reference proteome of brain capillary endothelial cells

Project description:Bovine brain capillary endothelial cells (BBCEC) are cocultured in presence of rat astrocytes to reinduce blood brain barrier properties. After checking of BBB propereties reinduction, BBCEC are harvested by collagenase treatment, lysed with Triton X-100 based lysis buffer. Triton X-100 soluble proteins are subjected to organic fractionation step and the 5 generated fractions were submitted to 1D-LC-MS/MS analysisi on MALDI-TOF-TOF-MS.

2010-12-03 | PRD000250 | Pride

HNSCC cell lines for CoGAPS matrix factorization algorithm identifies AP-2alpha as a feedback mechanism from therapeutic inhibition of the EGFR network

Project description:To determine the expression AP2-alpha target genes, global gene expression of 7 HNSCC cell lines with and without cetuximab treatment (100 nM, 24 hrs) and the HaCaT keratinocyte cell line was performed.

2017-06-12 | GSE62027 | GEO

Tscherskia triton

Project description:Tscherskia triton overview

| PRJNA816940 | ENA

Porcine LC-MSMS - Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

Project description:Porcine breast fatty extracellular matrix proetiens has been extracted after decellularization of fatty tissue with conventional method (PFT-1) using triton X-100 and sodium dodecyl sulfate and extracted with modified methods keeping natural protein in their natural condition using X-100, urea, thiourea etc

2019-11-12 | PXD011011 | Pride

Transcription profiling of Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant

Project description:Whole genome expression analyses between wild-type Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant. Expression analyses between these strains were conducted following growth under physiological conditions, or following exposure to non-inhibitory concentrations of SDS or Triton X-100.

2006-03-01 | E-MEXP-552 | biostudies-arrayexpress

Pseudomonas-derived Ceramidase Induces Production of Inflammatory Mediators from Human Keratinocytes via Sphingosine-1-Phosphate

Project description:A Pseudomonas aeruginosa-derived neutral ceramidase (PaCDase) isolated from a patient with atopic dermatitis was shown to effectively degrade ceramide in the presence of Staphylococcus aureus-derived lipids or neutral detergents. To understand the effect of ceramide metabolites on the functions of differentiating keratinocytes, we have conducted genes expressions analysis from PaCDase-, sphingosine-1-phosphate (S1P)-treated or untreated three-dimensionally cultured human primary keratinocytes, which form a stratum corneum, in the presence of Triton X-100 using high resolution DNA microarray. To evaluate the effects of PaCDase and S1P on keratinocyte functions, we applied an epicutaneous 24-h patch test on the EPI-Model in keratinocyte serum-free medium (keratinocyte-SFM) (Invitrogen; Carlsbad, CA, USA) by the method of Spiekstra et al. [25] with some modification. The Triton X-100 treated samples were labeled using Cy5, and the PaCDase or S1P samples were labeled with Cy3.

2014-12-25 | E-GEOD-53670 | biostudies-arrayexpress

Pseudomonas-derived Ceramidase Induces Production of Inflammatory Mediators from Human Keratinocytes via Sphingosine-1-Phosphate

2014-12-25 | GSE53670 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data