The surface proteome of extracellular vesicles from Fasciola hepatica

ABSTRACT: Helminth parasites secrete extracellular vesicles (EVs) as a means of exporting effector molecules into the host microenvironment. Once released, the parasite-derived EVs are internalised by host immune cells and trigger a range of biological effects including modulation of host immunity. While the molecular cargo of EVs have been characterised in many parasites, little is known about the surface-exposed molecules that may effect ligand-receptor interactions with the target host cell surface that initiate vesicle docking and subsequent internalisation. Here we used a membrane-impermeable biotin reagent to capture proteins displayed on the outer membrane surface of adult F. hepatica EVs and mass spectrometry to identified the surface proteins.

INSTRUMENT(S):

ORGANISM(S): Fasciola Hepatica (liver Fluke)

TISSUE(S): Protein Secretion, Secretory Cell

DISEASE(S): Fascioliasis

SUBMITTER: Mark Robinson  

LAB HEAD: Mark Robinson

PROVIDER: PXD007782 | Pride | 2019-11-12

REPOSITORIES: Pride