Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture
DISEASE(S): Brain Cancer
SUBMITTER: Patrick Murphy
LAB HEAD: Shashi Gujar
PROVIDER: PXD007790 | Pride | 2018-08-14
REPOSITORIES: Pride
Cell reports 20180801 9
NAD<sup>+</sup> is a key metabolic redox cofactor that is regenerated from nicotinamide through the NAD<sup>+</sup> salvage pathway. Here, we find that inhibiting the NAD<sup>+</sup> salvage pathway depletes serine biosynthesis from glucose by impeding the NAD<sup>+</sup>-dependent protein, 3-phosphoglycerate dehydrogenase (PHGDH). Importantly, we find that PHGDH<sup>high</sup> breast cancer cell lines are exquisitely sensitive to inhibition of the NAD<sup>+</sup> salvage pathway. Further, we fi ...[more]