Proteomics

Dataset Information

48

Proteomics of Nitrospirae - Rice paddy Nitrospirae encode and express genes related to sulfate respiration: proposal of the new genus Candidatus Sulfobium


ABSTRACT: We present the draft genome of Nitrospirae bacterium Nbg-4 as a representative of this clade and couple this to in situ protein expression under sulfate-enriched and sulfate-depleted conditions in rice paddy soil. The proteins were extracted from the soil and analysed via LC-MS/MS measurements.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bacteria  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Jana Seifert  

LAB HEAD: Jana Seifert

PROVIDER: PXD007817 | Pride | 2018-01-08

REPOSITORIES: Pride

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Publications

Rice Paddy Nitrospirae Carry and Express Genes Related to Sulfate Respiration: Proposal of the New Genus "Candidatus Sulfobium".

Zecchin Sarah S   Mueller Ralf C RC   Seifert Jana J   Stingl Ulrich U   Anantharaman Karthik K   von Bergen Martin M   Cavalca Lucia L   Pester Michael M  

Applied and environmental microbiology 20180214 5


Nitrospirae spp. distantly related to thermophilic, sulfate-reducing Thermodesulfovibrio species are regularly observed in environmental surveys of anoxic marine and freshwater habitats. Here we present a metaproteogenomic analysis of Nitrospirae bacterium Nbg-4 as a representative of this clade. Its genome was assembled from replicated metagenomes of rice paddy soil that was used to grow rice in the presence and absence of gypsum (CaSO4·2H2O). Nbg-4 encoded the full pathway of dissimilatory sul  ...[more]

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