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Pediatric IBD MLI metaproteomics

ABSTRACT: Mucosal-luminal interface (MLI) samples were collected from a cohort of children with new-onset IBD and microbial cells were harvested and processed for metaproteomic analysis. Deep metaproteomics data analysis was then performed for better understanding the MLI microbiota functions in the development of pediatric IBD.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Human Gut Metagenome

SUBMITTER: Xu Zhang

LAB HEAD: Daniel Figeys

PROVIDER: PXD007819 | Pride | 2018-08-03

REPOSITORIES: Pride

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Metaproteomics reveals associations between microbiome and intestinal extracellular vesicle proteins in pediatric inflammatory bowel disease.

Zhang Xu X Deeke Shelley A SA Ning Zhibin Z Starr Amanda E AE Butcher James J Li Jennifer J Mayne Janice J Cheng Kai K Liao Bo B Li Leyuan L Singleton Ruth R Mack David D Stintzi Alain A Figeys Daniel D

Nature communications 20180720 1

Alterations in gut microbiota have been implicated in the pathogenesis of inflammatory bowel disease (IBD), however factors that mediate the host-microbiota interactions remain largely unknown. Here we collected mucosal-luminal interface samples from a pediatric IBD inception cohort and characterized both the human and microbiota proteins using metaproteomics. We show that microbial proteins related to oxidative stress responses are upregulated in IBD cases compared to controls. In particular, w ...[more]

PMID: 30030445

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