Landscape of nuclear transport receptor cargo specificity

ABSTRACT: Nuclear transport receptors (NTRs) recognize localization signals of cargos to facilitate their passage across the central channel of nuclear pore complexes (NPCs). About 30 different NTRs constitute different transport pathways in humans and bind to a multitude of different cargos. The exact cargo spectrum of the majority of NTRs, their specificity and even the extent to which active nucleocytoplasmic transport contributes to protein localization remains largely unknown because of the transient nature of these interactions and the wide dynamic range of cargo concentrations. To systematically map cargo-NTR relationships in situ, we used proximity ligation coupled to mass spectrometry (BioID). We systematically fused the engineered biotin ligase BirA* to 16 NTRs. We estimate that at least one third of the human proteome is subject to active nuclear transport. We quantified the specificity and redundancy in cargo-NTR interactions, and identified transport pathways for various protein complexes and transcription factors. We extended the BioID method by the direct identification of biotinylation sites. This approach enabled us to identify interaction interfaces and to discriminate direct versus piggyback transport mechanisms.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Marie-Therese Mackmull  

LAB HEAD: Martin Beck

PROVIDER: PXD007976 | Pride | 2017-11-14

REPOSITORIES: Pride