Peptidomic Analysis of Hemorrhagic Shock Pig Plasma
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)
TISSUE(S): Blood Plasma
DISEASE(S): Cardiovascular System Disease
SUBMITTER: 
Elisa Maffioli
LAB HEAD: Gabriella Tedeschi
PROVIDER: PXD008018 | Pride | 2021-09-08
REPOSITORIES: Pride
Publications
High-Resolution Mass Spectrometry-Based Approaches for the Detection and Quantification of Peptidase Activity in Plasma.
Maffioli Elisa E Jiang Zhenze Z Nonnis Simona S Negri Armando A Romeo Valentina V Lietz Christopher B CB Hook Vivian V Ristagno Giuseppe G Baselli Giuseppe G Kistler Erik B EB Aletti Federico F O'Donoghue Anthony J AJ Tedeschi Gabriella G
Molecules (Basel, Switzerland) 20200906 18
Proteomic technologies have identified 234 peptidases in plasma but little quantitative information about the proteolytic activity has been uncovered. In this study, the substrate profile of plasma proteases was evaluated using two nano-LC-ESI-MS/MS methods. Multiplex substrate profiling by mass spectrometry (MSP-MS) quantifies plasma protease activity in vitro using a global and unbiased library of synthetic peptide reporter substrates, and shotgun peptidomics quantifies protein degradation pro ...[more]
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