Proteomics

Dataset Information

54

First dataset - bacterial proteome of carbapanemase-producing Escherichia coli and other Enterobacteriaceae under antibiotic pressure


ABSTRACT: Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such carbapenemase-producing Enterobacteriaceae (CPE) are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli, producing either NDM, IMP or KPC type carbapenemase were included in this study, and their proteomes were analysed in growth conditions with or without meropenem.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Escherichia coli  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Recurrent Urinary Tract Infections,Sepsis

SUBMITTER: Hanna Sidjabat  

LAB HEAD: Hanna Sidjabat

PROVIDER: PXD008019 | Pride | 2018-07-11

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications

The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure.

Sidjabat Hanna E HE   Gien Jolene J   Kvaskoff David D   Ashman Keith K   Vaswani Kanchan K   Reed Sarah S   McGeary Ross P RP   Paterson David L DL   Bordin Amanda A   Schenk Gerhard G  

Scientific reports 20180301 1


Antibiotic resistance associated with the clinically significant carbapenemases KPC, NDM and OXA-48 in Enterobacteriaceae is emerging as worldwide. In Australia, IMP-producing Enterobacteriaceae are the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such CPE are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specificall  ...[more]

Similar Datasets

2017-01-01 | S-EPMC5477705 | BioStudies
2020-01-01 | S-EPMC7160683 | BioStudies
2020-01-01 | S-EPMC7454053 | BioStudies
2018-01-01 | S-EPMC5832786 | BioStudies
1000-01-01 | S-EPMC5075087 | BioStudies
2019-01-01 | S-EPMC6658802 | BioStudies
2018-01-01 | S-EPMC5962079 | BioStudies
2019-01-01 | S-EPMC6375898 | BioStudies
2020-01-01 | S-EPMC7602311 | BioStudies
1000-01-01 | S-EPMC5366124 | BioStudies